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In your publication cite :
NPS@: Network Protein Sequence Analysis
TIBS 2000 March Vol. 25, No 3 [291]:147-150
Combet C., Blanchet C., Geourjon C. and Deléage G.

CLUSTALW multiple alignment

Abstract Thompson, J.D., Higgins, D.G. and Gibson, T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. (1994) Nucleic Acids Research, 22, 4673-4680 [HELP]
alignment with width :
residues.
Conservation level of % (works with "using conservation level of" option in list above, negative value will hide).

with percentage of secondary structure.

DSC (King and Sternberg, 1996) [HELP]
DPM (Deleage and Roux, 1987) [HELP]
GOR I (Garnier et al.,1978) Choose parameters [HELP]
GOR III (Gibrat et al.,1987) [HELP]
GOR IV (Garnier et al., 1996) [HELP]
HNN (Guermeur, 1997) [HELP]
SIMPA96 (Levin et al., 1996) [HELP]
PHD (Rost et al., 1994) [HELP]
PREDATOR (Argos et al., 1996) Choose parameters [HELP]
SOPM (Geourjon and Deleage, 1994) [Choose parameters] [HELP]

View CLUSTALW in: [AnTheProt (PC) , Download...] [HELP] 


                         10        20        30        40        50        60
                          |         |         |         |         |         |
frog_kiss2       MLLLLL---LTLVISQHAVGGTMFRGDEEGLELEEIGGPETSYPEG--------------
lizard_kiss      MIRLLFSSFLLAVICQNGTWGKPVHGDIR--SILQVDFSDAADPAN--------------
human_kiss1      -MNSLVSWQLLLFLCATHFGEPLEKVASVGNSRPTGQQLESLGLLA--------------
mouse_kiss1      -MISMASWQLLLLLCVATYGEPLAKVK----PGSTGQQSGPQELVN--------------
opposum_kiss     -MRSSVYWQLLLLLSVSPFGETSDKFAPVENPGRTGTSSLSQEASSSDFSALGQPGRLLA
frog_kiss1       -MSSLCLFLFLLGIHLGRSDHTAKNTDELYSQVPGKSQWLGSLLCP--------------
zfish_kiss2      ----MNTRALILFMSAMVSQSTAMRAILT--------DMDTPEPMP--------------
mackerel_kiss2   --MRLVALVVVCGLMLGHDGGSLAAALP---------GFDSEQRTQG-------------
zfish_kiss1      ----MMLLTVILMLSVARVHTNPSGHF----------QYYLEDETP--------------
mackerel_kiss1   -----MMLRLLVALTVAALSTEVYSTN----------SVKSTNYSE--------------
                          .   :                                              
Prim.cons.       MMRSLVS2QLLL4LSVA33G2TLAK4D2VGN2GPTGQQSDS3E22P2DFSALGQPGRLLA

                         70        80        90       100       110       120
                          |         |         |         |         |         |
frog_kiss2       ---DPREKSESYELIPSADTLSWPGRSNICYFIREGRLESQLSCHLRFTRSKFNFNPFGL
lizard_kiss      ---DFQAKRNSYHAAQGPEVLNPADPSSLCYLIQESDTESQISCRLRFTRSKFNFNPFGL
human_kiss1      ---PGEQSLPCTERKPAATARLSRRGTSLSPPPES--SGSPQQPGLSAPHSRQIPAPQGA
mouse_kiss1      ---AWEKESRYAESKPGSAGLRARR-SSPCPPVEG--PAGRQRP-LCASRSRLIPAPRGA
opposum_kiss     HLIPWEGRPQCLE-KPEQTGQTQRL-AMLCPSDE---ASDPLWPGLCPTRSRLITAPQGA
frog_kiss1       ---EKVPTTRRAEQMPVLSLLCRRK-KSLSTGHPW--STDSLLP------SRSISAPEGE
zfish_kiss2      ---DPKPRFLSME-RRQFEEPSASDDASLCFFIQEKDETSQISCKHRLARSKFNYNPFGL
mackerel_kiss2   ----RASVLSALR-RSSADYLEDPN---LCFSLRENDDQRQLLCNDR--RSNFNFNPFGL
zfish_kiss1      ----EETSLRVLR---GTDTRPTDG----SPPSK---LSALFSMGAGPQKNTWWWSPES-
mackerel_kiss1   ----DQVILKALRDLSHVSILPSTT---LSGNVP---ADKVQSADRKFPRARWWIPKVIL
                             .                .                              
Prim.cons.       HLID2EP3LRSLE6KPGAD2L22R34SSLCPPIEE4D33SQLS2GLRFTRSRF2F2PFGL

                        130       140       150       160       170
                          |         |         |         |         |
frog_kiss2       -RFGKRARG--DANGEGLAPLVPRRLLPFLLKLKDKR----CSESVGESC
lizard_kiss      -RFGKRQGGVLAADTRKLGPQSSSKMLHSLLKPKLDRIIQLCGQMWGEGC
human_kiss1      -VLVQREKDLPNYNWNSFG-------LRFGKREAAPGN-HGRSAGRG---
mouse_kiss1      -VLVQREKDLSTYNWNSFG-------LRYGRRQAAR-------AARG---
opposum_kiss     -LLVEREKDMSTYNWNSFG-------LRYGKRQTNRG------KARVPA-
frog_kiss1       -FLVQREKDLSTYNWNSFG-------LRYGKRGSGSE------NSKTKVW
zfish_kiss2      -RFGKRNEAT-TSDSDRLKHKHLLPMMLYLRKQLETS-------------
mackerel_kiss2   -RFGKRYNGYIYRRAVKRARTDQFTPVSLFPRELEVPT------------
zfish_kiss1      -PYTKRRQNVAYYNLNSFG-------LRYGKREQDMLTR---LKQKSPVK
mackerel_kiss1   PQTVKKHQDMSSYNFNSFG-------LRYGK-------------------
                     ::                    :                                 
Prim.cons.       PR2VKREKDLSTYNWNSFGP44444MLRYGKR233R2T222CS22RG2VC
Alignment data :
Alignment length : 170
Identity (*) : 0 is 0.00 %
Strongly similar (:) : 4 is 2.35 %
Weakly similar (.) : 3 is 1.76 %
Different : 163 is 95.88 %
Sequence 0001 : frog_kiss2 ( 143 residues).
Sequence 0002 : lizard_kiss ( 150 residues).
Sequence 0003 : human_kiss1 ( 138 residues).
Sequence 0004 : mouse_kiss1 ( 126 residues).
Sequence 0005 : opposum_kiss ( 149 residues).
Sequence 0006 : frog_kiss1 ( 129 residues).
Sequence 0007 : zfish_kiss2 ( 125 residues).
Sequence 0008 : mackerel_kiss2 ( 123 residues).
Sequence 0009 : zfish_kiss1 ( 116 residues).
Sequence 0010 : mackerel_kiss1 ( 105 residues).
CLUSTALW options used :
endgaps=1
gapdist=8
gapext=0.2
gapopen=10.0
hgapresidues=GPSNDQERK
matrix=gonnet
maxdiv=30
outorder=aligned
pwgapext=0.1
pwgapopen=10.0
pwmatrix=gonnet
type=PROTEIN
Result files (text) :
CLUSTALW
Garnier parameters

Auto calculated
Decision constants HelixSheetTurnCoil
Return


PREDATOR parameters

Secondary structure data
Return


SOPM parameters

Number of conformational states
Similarity threshold : Window width :
Return

SOPMA parameters

Number of conformational states
Similarity threshold : Window width :
Return

User : public@132.76.61.52. Last modification time : Mon May 30 07:53:52 2022. Current time : Mon May 30 07:53:52 2022