Introduction to Deep Sequencing Analysis course 2016

Date

Hours

Subject

Lecturer

March 13th

09:00-10.45

Introduction to Illumina NGS technology

Shmulik Motola

11:00-12:00

NGS applications and introduction to analysis

Esti Feldmesser

12:00-12:30

Lunch

12:30-13:30

Illumina Primary analysis Pipeline and Quality Control, Sequence alignment to genome

Dena Leshkowitz & Esti Feldmesser

13:30-14:30

Exercise 1: Chipster for QC and Mapping

Dena Leshkowitz & Esti Feldmesser

March 14th

09:00-10:15

Introduction to RNA-Seq analysis gene expression analysis (HTSeq, DESeq)

Dena Leshkowitz

Introduction to RNA-Seq analysis VIDEO

10:30-12:15

Exercise 2: Learn how to detect differentially expressed genes from RNA-Seq data with Chipster

Dena Leshkowitz & Naama Kopelman

12:15-12:45

Lunch

12:45-13:15

Clustering

Gilgi Friedlander

Clustering VIDEO

13:15-14:30

Exercise 3: Clustering

Gilgi Friedlander & Naama Kopelman

March 15th

09:00-10:00

RNA-Seq transcript level analysis

Dena Leshkowitz

10:00-11:15

Exercise 4: Using Chipster for transcript assembly

Dena Leshkowitz & Esti Feldmesser

11:30-13:00

Functional analysis: Gene Ontology and pathways

Esti Feldmesser

13:00-13:30

Lunch

13:30-14:30

Exercise 5: Functional analysis for animals and plants: David and ThaleMine, Tools table

Esti Feldmesser & Naama Kopelman

March 16th

09:00-10:00

Advanced functional analysis: Gene Set Analysis (GSEA) and Ingenuity; Exercise Input

Esti Feldmesser

Advanced functional analysis VIDEO

10:15-11:00

Demo: Ingenuity, Exercise 6: GSEA

Esti Feldmesser & Dena Leshkowitz

11:15-12:30

ChIP-Seq: Using NGS to Discover Protein-DNA Interactions

Dena Leshkowitz

ChIP-Seq: Using NGS VIDEO

12:30-13:00

Lunch

13:00-14:30

Exercise 7: ChIP-Seq Analysis

Dena Leshkowitz & Esti Feldmesser

March 17th

09:00-9:15

Starting Final Exercise

Esti Feldmesser

09:15-10:30

Variant detection

Barak Markus

Variant detection VIDEO

10:45-12:00

Final Exercise

Esti Feldmesser & Dena Leshkowitz & Gilgi Friedlander

12:00-12:30

Lunch

12:30-13:30

Introduction to single-cell transcriptomics, Fluidigm platform for Single Cell Genomics

Esti Feldmesser & David Pilzer

Single Cell Genomics VIDEO

13:30-14:30

Course Summary: Questions & Answers

Dena Leshkowitz

Course Summary: Questions & Answers VIDEO